In a monohybrid cross Tt × Tt — draw the Punnett square and find ratios

easy CBSE NEET 3 min read

Question

In a monohybrid cross between two heterozygous tall plants (Tt×TtTt \times Tt), draw the Punnett square and determine the phenotypic and genotypic ratios of the offspring.

Solution — Step by Step

Both parents are heterozygous tall plants with genotype TtTt.

TT = dominant allele (tall) tt = recessive allele (dwarf)

Each parent produces two types of gametes: TT and tt (in equal proportions).

Arrange parent gametes along the top and side of the square:

Tt
TTTTt
tTttt

The four boxes represent the four equally likely offspring genotypes.

From the Punnett square:

  • TTTT: 1 box (25%)
  • TtTt: 2 boxes (50%)
  • tttt: 1 box (25%)

Genotypic ratio: 1 TT : 2 Tt : 1 tt

  • TTTT → Tall (homozygous dominant): 1 plant
  • TtTt → Tall (heterozygous, but T is dominant): 2 plants
  • tttt → Dwarf (homozygous recessive): 1 plant

Phenotypic ratio: 3 Tall : 1 Dwarf

Why This Works

This is Mendel’s Law of Segregation in action. Each parent has two alleles for height (TtTt), and these alleles separate during gamete formation (meiosis). Each gamete receives exactly one allele. At fertilization, alleles come together randomly — giving the 1:2:1 genotypic ratio.

The 3:1 phenotypic ratio arises because TT is dominant — it masks the expression of tt. Whether a plant has one or two copies of TT, it looks tall. Only plants with two copies of tt (homozygous recessive) appear dwarf.

This 3:1 ratio is what Mendel observed in his pea plant experiments, and it was the experimental foundation of genetics.

Alternative Method — Probability Approach

Instead of a Punnett square, use probability:

  • P(T from parent 1)=12P(T \text{ from parent 1}) = \frac{1}{2}, P(t from parent 1)=12P(t \text{ from parent 1}) = \frac{1}{2}
  • Same for parent 2

P(TT)=12×12=14P(TT) = \frac{1}{2} \times \frac{1}{2} = \frac{1}{4}

P(Tt)=P(Tf)+P(tT)=14+14=12P(Tt) = P(Tf) + P(tT) = \frac{1}{4} + \frac{1}{4} = \frac{1}{2}

P(tt)=12×12=14P(tt) = \frac{1}{2} \times \frac{1}{2} = \frac{1}{4}

Genotypic ratio: 14:12:14=1:2:1\frac{1}{4} : \frac{1}{2} : \frac{1}{4} = 1:2:1

Phenotypic ratio: 34\frac{3}{4} tall : 14\frac{1}{4} dwarf = 3:1

Common Mistake

Students sometimes give only the genotypic ratio and forget the phenotypic ratio (or confuse the two). The question usually asks for BOTH. Genotypic ratio = 1:2:1 (three distinct genotypes). Phenotypic ratio = 3:1 (only two visible types, since TT and Tt both look tall). These are different answers and must be stated separately.

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